鸡ev21占位区和未占位区重复序列结构与羽速表型关系研究

doi:10.11843/j.issn.0366-6964.2017.06.003

Abstract

Abstract:

The aim of this study was to explore the molecular basis of Hae Ⅲ digestion detection of genotypes of chicken feather speed in chicken. Three hundred and thirteen individuals in 6 strains of chickens (Taihang, Bashang Long-tail, Dawufen, Hy-line Gray, Hy-line Brown, progenitor of Hy-line Gray) with definite phenotype of feather speed were detected with RFLP. The common region of occupied site(OS) and unoccupied site(US) for 538 bp, also the specific region of OS for 1 440 bp, were digested with Hae Ⅲ. The results showed that: 1) The fragment for 1 450 bp was the unoccupied-site repeated sequence for ev21 ascertained by Blast. The digestion detection results of Hae Ⅲ for 1 450 bp were completely consistent with the phenotype of HY-line Gray and progenitors of HY-line Gray and Dawufen, but their consistency with Taihang slow-feather cock, Taihang slow-feather hen, Bashang Long-tail slow-feather cock and HY-line Brown slow-feather chicken were 40.0%, 27.6%, 28.6% and 0.0%, respectively. 2) The consistency was above 92% between digestion results of 538 bp and phenotype in Taihang and Bashang Long-tail chicken, and the other strains were 100.0% except for HY-line Brown fast-feather cock with 0.0%. 3) The positive rate of 1 440 bp fragment PCR in slow-feather Taihang cock, Taihang hen and fast-feather Taihang cock was 94.1%, 65.5% and 0.0%, respectively, and it was 100.0% and 0.0% in slow-feather and fast-feather progenitors of Hy-line gray, respectively. The 1 440 bp fragment could not be digested by Hae Ⅲ and it could be a detection basis for ev21.The structure was analyzed comprehensively for the OS and US of ev21 in 6 strains of chicken and it was concluded that the site recognized by Hae Ⅲ enzyme in US could not be a detection base for slow-feather and ev21, while ev21 insertion in OS and the site for Hae Ⅲ digestion in 1 440 bp were linked with the mutation of A → G and 8 bases repeated.

CLC Number:

S831.2

ZHANG Le-chao, WANG Han, ZHANG Xiu-ling, LIU Chun-yang, WANG Qi, ZHOU Rong-yan, LI Xiang-long, LI Lan-hui. Relation between Feather Speed and Gene Structure of Repeated Sequences of ev21 Occupied-site and Unoccupied-site Regions in Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 990-999.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.06.003

https://www.xmsyxb.com/EN/Y2017/V48/I6/990

References

[1] SEREBROVSKY A S. Crossing-over involving three sex-linked genes in chickens[J]. Am Nat, 1922, 56(647):571-572.
[2] SOHN S H, KIM N Y, PARK D B, et al. Influence of early- and late-feathering phenotype on productive performance in the feather-sexing strains of Korean native chicken[J]. Korean J Poult Sci, 2013, 40(3):263-270.
[3] SOHN S H, PARK D B, SONG H R, et al. Genotype frequencies of the sex-linked feathering and their phenotypes in domestic chicken breeds for the establishment of auto-sexing strains[J]. J Anim Sci Technol, 2012, 54(4):267-274.
[4] LUO C L, SHEN X, RAO Y S, et al. Differences of Z chromosome and genomic expression between early- and late-feathering chickens[J]. Mol Biol Rep, 2012, 39(5):6283-6288.
[5] ZHAO J, YAO J, LI F, et al. Identification of candidate genes for chicken early- and late-feathering[J]. Poult Sci, 2016, 95(7):1498-1503.
[6] BACON L D, SMITH E, CRITTENDEN L B, et al. Association of the slow feathering (K) and an endogenous viral (ev21) gene on the Z chromosome of chickens[J]. Poult Sci, 1988, 67(2):191-197.
[7] KANSAKU N, GUÉMENÉ D, NAKAMURA A, et al. Sequence characterization of K-gene linked region in various chicken breeds[J]. J Poult Sci, 2011, 48(3):181-186.
[8] TIXIER-BOICHARD M H, BENKEL B F, CHAMBERS J R, et al. Screening chickens for endogenous virus ev21 viral element by the polymerase chain reaction[J]. Poult Sci, 1994, 73(10):1612-1616.
[9] LEVIN I, SMITH E J. Molecular analysis of endogenous virus ev21-slow feathering complex of chickens.:1. Cloning of proviral-cell junction fragment and unoccupied integration site[J]. Poult Sci, 1990, 69(11):2017-2026.
[10] LEVIN I, SMITH E J. Association of a chicken repetitive element with the endogenous virus-21 slow-feathering locus[J]. Poult Sci, 1991, 70(9):1948-1956.
[11] IRAQI F, SMITH E J. Determination of the zygosity of ev21-k in late-feathering male white leghorns using the polymerase chain reaction[J]. Poult Sci, 1994, 73(7):939-946.
[12] ELFERINK M G, VALLéE A A, JUNGERIUS A P, et al. Partial duplication of the PRLR and SPEF2 genes at the late feathering locus in chicken[J]. BMC Genomics, 2008, 9(1):391.
[13] 李培周, 朱晓萍, 邝智祥, 等. 清远麻鸡羽速基因的分子检测及其与体重和冠高相关性分析[J]. 中国畜牧杂志, 2013, 49(7):10-12.
LI P Z, ZHU X P, KUANG Z X, et al. Molecular detection of feather speed and relation between crown height and body weight Qingyuan Partridge chickens[J]. Chinese Journal of Animal Science, 2013, 49(7):10-12. (in Chinese)
[14] 李培周, 李华, 杜炳旺, 等. 贵妃鸡羽速基因分子检测及相关早熟性状分析[J]. 中国家禽, 2013, 35(21):5-8.
LI P Z, LI H, DU B W, et al. Molecular detection of feathering locus and prematurity traits of princess chicken[J]. China Poultry, 2013, 35(21):5-8. (in Chinese)
[15] 李珊珊, 李东华, 吕福琨, 等. 慢羽系麒麟公鸡纯合个体分子检测方法的建立[J]. 河南农业科学, 2016, 45(7):118-121.
LI S S, LI D H, LÜ F K, et al. Establishment of molecular identification techniques for frizzle chicken early feathering and late feathering[J]. Journal of Henan Agricultural Sciences, 2016, 45(7):118-121. (in Chinese)
[16] 李竞一, 李荣妮, 王晓亮, 等. 慢羽鸡ev21结合位点缺失个体的检测[J]. 中国畜牧杂志, 2011, 47(11):6-8.
LI J Y, LI R N, WANG X L, et al. The detection for individuals without integration locus of ev21 in slow feather chickens[J]. Chinese Journal of Animal Science, 2011, 47(11):6-8. (in Chinese)
[17] SMITH A, BENKEL B F. Novel avian leukosis virus-related endogenous proviruses from layer chickens:characterization and development of locus-specific assays[J]. Poult Sci, 2009, 88(8):1580-1585.
[18] 陈忠, 彭秀丽, 李世军, 等. 鸡快慢羽分子鉴定方法的建立及其应用[J]. 中国家禽, 2010, 32(4):11-13.
CHEN Z, PENG X L, LI S J, et al. Establishment of the molecular approach to identify chicken's early/late feathering phenotype and its application[J]. China Poultry, 2010, 32(4):11-13.
[19] BU G X, HUANG G A, FU H, et al. Characterization of the novel duplicated PRLR gene at the late-feathering K locus in Lohmann chickens[J]. J Mol Endocrinol, 2013, 51(2):261-276.

Relation between Feather Speed and Gene Structure of Repeated Sequences of ev21 Occupied-site and Unoccupied-site Regions in Chicken

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TANG Xinxin, ZHENG Jumei, LUO Na, YING Fan, ZHU Dan, LI Sen, LIU Dawei, AN Bingxing, WEN Jie, ZHAO Guiping, LI Hegang. Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 99-109.
[2]	XU Xiaojing, DONG Ruiling, WEI Limin, ZHAO Shaomeng, ZHAO Guiping, ZHANG Minhong, FENG Jinghai. Study on Growth Model of Wenchang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4952-4961.
[3]	QI Lina, LU Xuelin, YANG Kaixuan, ZHOU Jin, LI Xianyu, LIU Ke, ZHANG Wengang, GU Xin, ZHANG Weijian. Analysis of Genetic Diversity and Genetic Structure of New Pudong Chicken Based on SNP Chips [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4962-4971.
[4]	WANG Yanxing, ZHANG Yushi, JI Haigang, LIU Yang, NIU Yufang, HAN Ruili, LIU Xiaojun, TIAN Yadong, KANG Xiangtao, LI Zhuanjian. Establishment and Analysis of the Chicken Skeletal Satellite Cell Line [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4972-4981.
[5]	FAN Chenyu, SHAN Yanju, ZHANG Ming, JI Gaige, JU Xiaojun, TU Yunjie, HE Xi, SHU Jingting, LIU Yifan, ZHANG Haihan. Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4982-4992.
[6]	CHEN Cheng, QIAO Xibo, SUN Yi, KANG Li, JIANG Yunliang. Study on the Polymorphism and Genetic Effect of the -868 Locus of FSHR Gene on Egg Laying Traits in Langya Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4560-4568.
[7]	JIN Sihua, JIA Fumin, HE Peili, JIA Yuqing, SHUI Fei, LIU Xuling, LIU Xing, WANG Xin, XU Man, GENG Zhaoyu. Effect of IHH Gene Overexpression on Proliferation and Differentiation of Chicken Primary Chondrocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4569-4576.
[8]	CHEN Xuejiao, LIU Huijie, ZANG Lei, FENG Jing, PENG Da, ZHANG Hao. Transcriptome Data from Chicken Embryo Heart Tissue Identified Key Genes for Altitude Hypoxia Adaptation in Xueyu White Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4154-4163.
[9]	ZHANG Yinliang, YUE Qiaoxian, HUANG Chenxuan, CHEN Hui, WANG Dehe, ZHOU Rongyan. Construction of Tibia Transcript Profile of Laying Hens at the Early and Late Laying Stages and Analysis of Genes Related to Bone Metabolism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4164-4173.
[10]	ZHENG Gang, LIAN Ling. Research Progress of Key Regulatory Gene DMRT1 in Chicken Sex Determination and Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3152-3163.
[11]	BAI Lu, WANG Mengjie, MA Xiaochun, HE Zhengxiao, TAN Xiaodong, LIU Jie, ZHAO Guiping, WEN Jie, LIU Ranran. A Method for Quickly Mining the Characteristic SNP Markers Set of Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3252-3261.
[12]	CHEN Chun, KANG Zhaofeng, WEI Yue, LI Guanhong, WU Yanping, XIE Jinfang. Correlation of Wnt3a Gene Polymorphism with Skin Follicle Traits in Chongren Partridge Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2810-2823.
[13]	LIU Hang, WANG Huanhuan, GE Ying, ZHANG Lei, ZHANG Weiwu, WEI Yinghui, LI Qinghai, FAN Jinghui, ZHANG Xuedong. Screening of Candidate Genes of Skin Color of Black-Bone Chicken Based on Transcriptome and Proteome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2320-2329.
[14]	WANG Hui, XU Mengyuan, LIU Lingkang, ZHENG Xibang, LI Gonghe, WU Wende. PhiC31 Integrase Triggers Site-specific Cassette Exchange in Chicken Fibroblast Cells through Electroporation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2330-2342.
[15]	WANG Zheng, GUO Wenjie, CHENG Jin, YUAN Yitong, LUO Rong, XUE Yi, ZHANG Lihuan, ZHU Zhiwei, LI Huifeng. Association Study of Polymorphism in the Regulatory Region of Nutrient Transport-related Genes and Feed Conversion Ratio in Yellow-feather Broiler [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2343-2352.